{"version":"1.0","provider_name":"Proyecto Harmonia SYM","provider_url":"https:\/\/sym-prometeo2024-harmonia.es\/en\/","author_name":"admin","author_url":"https:\/\/sym-prometeo2024-harmonia.es\/en\/author\/sympromet23\/","title":"HOME - Proyecto Harmonia SYM","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"vJG6mfueDm\"><a href=\"https:\/\/sym-prometeo2024-harmonia.es\/en\/\">HOME<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/sym-prometeo2024-harmonia.es\/en\/embed\/#?secret=vJG6mfueDm\" width=\"600\" height=\"338\" title=\"&#8220;HOME&#8221; &#8212; Proyecto Harmonia SYM\" data-secret=\"vJG6mfueDm\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script>\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/\/# sourceURL=https:\/\/sym-prometeo2024-harmonia.es\/wp-includes\/js\/wp-embed.min.js\n<\/script>\n","description":"HARMONIA project Funded by Conseller\u00eda de Innovaci\u00f3n, Universidades, Ciencia ySociedad Digital de la Generalitat Valenciana (Ref. PROMETEU\/2020\/094). Who we are? The HARMONIA project pioneers the development of next-generation hydrogel-based wearable optical biosensors designed for continuous, non-invasive health monitoring. Leveraging advanced materials science, biofunctionalization, and optical biosensing technologies, HARMONIA aims to revolutionize personalized healthcare by transitioning from traditional, centralized diagnostics to home-based, real-time health monitoring solutions. At the core of this innovation are hydrogel-based wearable optical biosensors (HWOB) that utilize slide-ring hydrogels combined with specific bio-recognition probes. These sensors detect biomarkers in sweat and interstitial fluid (ISF) by responding to recognition events with measurable changes in volume and diffractive properties. Integrated with microneedles and holographic detection techniques, these skin patch biosensors offer precise, automated analysis. HARMONIA focuses on three critical healthcare applications: Inflammatory Bowel Disease (IBD) Monitoring: Continuous detection of biomarkers like C-reactive protein (CRP), Calprotectin (CP), and Interleukin-1\u03b2 (IL-1\u03b2) for early identification of disease flare-ups. Drug Allergy Testing: Innovative detection of allergen-specific immunoglobulin E (IgE) for safer, faster drug allergy diagnostics. Detection of Circulating Biomarkers: Unobtrusive tracking of health indicators such as microRNA, alpha-fetoprotein, and histamine linked to chronic and acute diseases. This groundbreaking research aims to enhance diagnostic precision, support proactive healthcare strategies, and contribute to achieve the United Nations Sustainable Development Goal 3 (Good Health and Well-being). HARMONIA promises transformative impacts in healthcare by enabling continuous, personalized monitoring that empowers clinicians and patients alike. Members Main Researchers Mar\u00eda Jos\u00e9 Ba\u00f1uls Polo Full Professor \u00c1ngel Maquieira Catal\u00e1 Emeritus Full Professor Members Luis Antonio Tortajada Genaro Professor Sergi Be\u00f1at Morais Ezquerro Professor Miguel \u00c1ngel Gonz\u00e1lez Mart\u00ednez Professor Jos\u00e9 Miguel Avell\u00e1 Oliver Associate Professor Mar\u00eda Victoria Gonz\u00e1lez Pedro Associate Professor Prometeo working group Mar\u00eda Isabel Luc\u00edo Benito Assistant Professor Aitor Cubells G\u00f3mez Predoctoral Researcher Pamela Abigail Chillagana Pilaplanta Predoctoral Researcher Josu\u00e9 Lombeyda Manzo Predoctoral Researcher Project Harmonia Project The project is structured into five Milestones, comprising nine Research Lines: MILESTONE 1 Study and testing of systems for biosensing based on holographic phenomena. This milestone is devoted to the development of basic analyte receptors employed in the biosensor systems, and is composed by two research lines: Research line 1: Hydrogel materialsResearch about development of new hydrogel materials, based on slide-rings, and their functionalization with nucleic acids and proteins is now in progress. Research line 2: Bioreagents and materials preparation by recombinant techniques.We aim the recombinant production of both specific IgE and peptides that mimic the major determinants of amoxicillin, as the main \u03b2-lactam antibiotics implicated in drug allergy. Also, development of bioactive M13 hydrogels tailored to interact with the target analyte, by expressing particular peptides on the surface of the phage M13, is under study. J Incl Phen Macro Chem, 2020, 98, 43 Talanta, 2024, 279, 126563 MILESTONE 2 Transducers Gratings ol materials optimized in Milestone 1 will be fabricated through different approaches: creating biogratingsn the hydroge of photoactivatable hydrogels, recording gratings by holography in transmission and reflection modes with self-polymerizable monomers, as well as water-stabilized perovskites. All the transducers are to be evaluated in their sensing capacity. Three research lines are to be explored. Research line 3. Biogratings.Photoactivatable hydrogel biogratings are being designed and further evaluated. Research line 4. Volume hologram gratings (VHG). To be fabricated on slide-ring hydrogels and using water-stable perovskites as recording material. Research line 5. Transducers made of M13 hydrogels. Whose design, optimization and performance analysis comprises the following activities: concentration-mediated self-organization and gelation; characterization of M13 hydrogels; effect of cross-linkers; and study of emission change upon target exposure. Biosens Bioelectron, 2021, 193, 113561Biosens Anal Chim Acta, 2018, 1033, 173 Anal Bioanal Chem, 2022, 2, 993 10 ACS Applied Materials &amp; Interfaces, 2024, 16, 48187 Gels, 2023, 9, 710 Gels, 2023, 9, 710 MILESTONE 3 Synthesis, characterization and functionalization of perovskite nanocrystals for biosensing applications Different approaches will be assessed and designed for fabricating wearable systems to collect and analyze sweat or ISF. Research line 6. Skin-patches for sweat collection. They will be designed and fabricated with a connected sampling and sensing cell. The performance and features of the final hydrogel-based optical transducer (VHG\/ biogratings) will be taken into consideration when comparing and choosing various designs. Research line 7.Microneedles for interstitial fluid collection. Microneedles will be moulded with hydrogels. Either, commercial hollow microneedles can be used. Applied Materials Today, 2023, 31, 101775 Proc Nat Ac Sci, 2018, 115, 4583 MILESTONE 4 Integration This milestone tackles to integrate the developments done in microneedles and in the transducers, to have a whole system, including, in the case of need, the reader system Research line 8. Sampling + Biosensing integration. Volume holographic gratings will be incorporated on hydrogel microneedles for ISF sampling and analysis. Hollow microneedles will be filled with developed M13 and DNA hydrogels for drug allergy testing and biomarkers detection, respectively. MILESTONE 5 Demonstrators The objective of this milestone is to demonstrate the functionality, safety, and analytical performances of the integrated biosensor developed. It aims to analyze the different solutions found to determine the one\/s with the highest potential and then integrating the developments done in microneedles and in the transducers, to have a whole system, including, in the case of need, the reader system. In an initial step, the prototype will be tested with artificial sweat, ISF and pork skin under laboratory conditions as a preliminary stage before the test in human skins. Research line 9. Proofs of concept. The following systems, coming from the integration of all developed technologies, will be set up and tested. Skin-patch with VHG\/biogratings for IBD biomarkers detection in sweat. Label-free hydrogel-based microneedle patch and VHG for IBD biomarkers in ISF. M13-based microneedle patch for allergy detection in ISF. Nucleic acid-hydrogel-based patch for detecting circulating biomarkers in ISF. Label-free hydrogel-based Microneedle patch and VHG for other biomarkers in ISF. Scientific production Publications All Publicaciones Drying-Induced Salt Deposition Patterns as a Tool for Label-Free Protein Quantification Drying-Induced salt deposition patterns as a tool for label-free protein quantification Arturo Patrone-Garc\u00eda, Miquel Avell\u00e0-Oliver, \u00c1ngel Maquieira Biosensors, 2025, 15,&#8230; Read more Advanced Hydrogel-based volume Holographic","thumbnail_url":"https:\/\/sym-prometeo2024-harmonia.es\/wp-content\/uploads\/elementor\/thumbs\/prometeo-quienessomos-r0vih67pyy67z6b42zce1ivdqptd1m8yem6suhqmwg.jpg"}